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KMID : 0387720080190020120
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Korean Journal of Blood Transfusion
2008 Volume.19 No. 2 p.120 ~ p.131
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Comparison of Cryopreservation Methods of Rare Red Blood Cells Used for Antibody Identification Tests
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Seo Ji-Weon
Han Kyou-Sup
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Abstract
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Background: When a certain antibody, which is not clearly identified, is detected before transfusion, rare red blood cells (RBCs) should be used for antibody identification. But its rarity make it difficult to identify the antibody. This study compared the high glycerol method with the liquid nitrogen method for the cryopreservation of rare RBCs that are used for antibody identification.
Methods: The frozen RBCs were thawed every 2 months to measure the strength of agglutination. We observed the changes of the strength of agglutination. The Dia antigen, which is relatively frequent in Asians, was included in this study.
Results: Using the high glycerol method, the decrease of the strength of agglutination was observed with using the k antigen from 4 months and the decrease of the strength of agglutination was observed with using M, N, s, Lea, Leb and Fyb antigens from 8 months. With freezing the donor RBCs by the liquid nitrogen method, the decrease of the strength of agglutination was observed with using the C, s, k and Leb antigens from 2 months, with using the M and S antigens from 4 months, with using the D, c, e, N and Fya antigens from 6 months and with using the Lea antigen from 8 months. Rare RBCs with the Dia antigen were successfully cryopreserved for 6 months with using the high glycerol method. For the commercial screening cells, the high glycerol method showed effective cryopreservation with using c, e, M, k, Lea, Leb, Jka and Fyb antigens for 6 months or longer.
Conclusion: We were able to preserve most of the important antigens of the RBCs for at least 6 months without a significant decrease of reactivity by employing the high glycerol cryopreservation technique.
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KEYWORD
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Cryopreservation, Rare red blood cells, High glycerol method, Liquid nitrogen method, Antibody identification
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